Ekinakozit PC3 androjen bağımsız prostat kanseri hücrelerinde hücre proliferasyonunu azaltır ve hücre invazyonunu inhibe eder

Purpose: The aim of this study was to determine the effects Echinacoside on cell proliferation, invasion and mRNA expression changes invasion-related genes in PC3 androgen-independent prostate cancer cells.
 Material Methods: effect proliferation cells determined by XTT method. Anti-invasive efficacy achieved using transwell chamber. Total RNA isolation performed Trizol cDNA subsequently synthesized. MMP2,MMP9, TIMP1, TIMP2 TIMP3 were also RT-PCR with SYBER Green.
 Results: In study, IC50 dose as 55.21 μM at 48th hour. It that echinacoside inhibited reduced 66% group. addition, it found statistically significant increased TIMP 1 1.96 times, 2.60 while decreasing MMP2 3.82 times MMP9 1.54 times.
 Conclusion: conclusion, revealed has an anti-proliferative cells. been shown can suppress regulating changes. With preliminary data presented terms detailed molecular biological studies be carried out its cancer.